I can show why a fire from which there is much smoke is better than one which burns with a clear flame, by a simple experiment. Here is a piece of gum benzoin, the substance from which Friar's balsam is made. This will burn, if we light it, just as tar burns, and without much smoke or smell. If, instead of burning it, we put some on a spoon and heat it gently, much more smoke is produced, and a fragrant scent is given off. In the same way we can burn spirit of lavender or eau de Cologne, but we get no scent from them in this way, for the burning destroys the scent. This is a very important fact in the disinfection of the air. The less the flame and the larger the quantity of smoke, the greater the effect produced, so far as disinfection is concerned. As air is a vapor, we must use our disinfectants in the form of vapor, so that the one may mix with the other, just as when we are dealing with fluids we must use a fluid disinfectant.

The question that presents itself is this: Can we so diffuse the vapor of an antiseptic like carbolic acid through the air as to destroy the germs which are floating in it, and thus purify it, making it like air which has been filtered through wool, or like that on the top of a lofty mountain? If the smoke of a wood fire seems to act as an antiseptic, and putrefaction is prevented, it seems reasonable to conclude that air could be purified and made antiseptic by some proper and convenient arrangement. Let us endeavor to test this by a few experiments.

Here is a large tube 6 inches across and 2 feet long, fixed just above a small tin vessel in which we can boil water and keep it boiling as long as we please. If we fill the vessel with carbolic acid and water and boil it very gently, the steam which rises will ascend and fill the tube with a vapor which is strong or weak in carbolic acid, according as we put more or less acid in the water. That is to say, we have practically a chimney containing an antiseptic vapor, very much the same thing as the smoke of a wood fire. We must be able to keep the water boiling, for the experiment may have to be continued during several days, and during this time must be neither stronger nor weaker in carbolic acid, neither warmer nor colder than a certain temperature. This chimney must be always at the same heat, and the fire must therefore be kept constantly burning. This is easily accomplished by means of a jet of gas, and by refilling the vessel every 24 hours with the same proportions of carbolic acid and water.

The question arises, how strong must this vapor be in carbolic acid to act as an antiseptic? It is found that 1 part acid to 50 of water is quite sufficient to prevent putrefaction. If we keep this just below boiling point there will be a gentle and constant rising of steam into the cylinder, and we can examine this vapor to see if it is antiseptic. We will take two test tubes half filled with water and put a small piece of beef into each of them and boil each for half a minute. One test tube we will hang up inside the cylinder, so that it is surrounded by carbolic acid vapor. The other we stand up in the air. If the latter is hung in a warm room, decomposition will soon take place in it; will the same thing happen to the other cylinder? For convenience sake we had best put six tubes inside the cylinder, so that we can take one out every day for a week and examine the contents on the field of a microscope. It will be necessary to be very particular as to the temperature to which the tubes are exposed, and the rates of evaporation beneath the cylinder.

I may mention that on some of the hottest days of last summer I made some experiments, when the temperature both of the laboratory and inside the cylinder was 75°F. I used test tubes containing boiled potatoes instead of meat, and found that the tube in the air, after 48 hours, abounded not simply with bacteria and other small bodies present in decomposition, but with the large and varied forms of protozoa, while the tube inside the cylinder contained no signs of decomposition whatever. When the room was cold the experiments were not so satisfactory, because in the former case there was very little if any current of air in the cylinder. This leads us to the question, why should we not make the solution of carbolic acid and water, and heat it, letting the steam escape by a small hole, so as to produce a jet? It is a singular fact that for all practical purposes such a steam jet will contain the same proportion of acid to water as did the original solution. The solution can of course be made stronger or weaker till we ascertain the exact proportion which will prevent decomposition.

From this arises naturally the question, what quantity of vapor must be produced in a room in order to kill the bacteria in its atmosphere? If we know the size of the room, shall we be able tell? These questions have not yet been answered, but the experiments which will settle them will be soon made, I have no doubt, and I have indicated the lines upon which they will be made. I have here a boiler of copper into which we can put a mixture, and can get from it a small jet of steam for some hours. A simple experiment will show that no bacteria will exist in that vapor. If I take a test tube containing meat, and boil it while holding the mouth of it in this vapor, after it has cooled we close the mouth with cotton wool, and set it aside in a warm place; after some days we shall find no trace of decomposition, but if the experiment is repeated with water, decomposition will soon show itself. Of course, any strength of carbolic acid can be used at will, and will afford a series of tests.

There are other methods of disinfecting the atmosphere which we cannot consider this evening, such as the very potent one of burning sulphur.

In conclusion, the lecturer remarked that his lecture had been cast into a suggestive form, so as to set his audience thinking over the causes which make the air impure, and how these impurities are to be prevented from becoming deleterious to health.