1. Into the anterior lymph sac of a frog inject 0.3 mil Tincture Aconite, diluted with equal amount normal saline. Observe effect on the various reflexes.

2. Expose the heart of a pithed frog; secure normal tracings on both the slow and fast drum. Into the thigh lymph sac inject 0.3 mil Tincture Aconite diluted. Continue tracings on slow drum, with an occasional cardiac curve on fast drum, noting carefully any variations in the graph, or in the physical condition of the heart.

3. Select two fasting rabbits, of equal weight and condition. Obtain normals of respiration and heart-rate. Then to one administer by stomach 0.1 mil x kg. Tincture Aconite; to the other give hypodermically o. 1 mg. x kg. Aconitine. Continue regular observations for several hours.

4. Select a large healthy cat or rabbit. Etherize carefully; connect with recording apparatus, and secure usual normals, including temperature. Continuing the records, slowly administer by vein a 20% dilution in warm Ringer's solution of Tincture Aconite. Keep account of amount of drug used, and note gradual accumulation of effect. Continue until animal dies.

5. Place a drop of Tincture Aconite on the tongue, and note effect.

6. On a student secure normals of respiration, pulse phenomena (rate, volume, energy), blood-pressure, and temperature. Administer orally 0.6 mil Tincture Aconite. Continue observations.

7. Although the use of Aconite has become markedly restricted, it may occasionally be studied in the wards of acute respiratory disorders. Particular attention should be paid to any registerable effect discoverable in relation to the circulation.

8. Look up recent literature, and make a brief presentation of the arguments for and against the use of Aconite.

Experiment No. i. Describe the effects of Aconite on a frog:

Experiment No. 2. Kymograph record of Aconite action on frog:

Experiment No. 3.

No. Date

Pharmacodynamics of Aconite Dose Administration

{ Rabbit (red = Tinct. Acon.

Subject { Rabbit (blue = Aconitine Age Sex Weight Condition

Temperature Humidity Reporter

Aconite 47

Experiment No. 4. Kymograph records: Tincture Aconite, intravenously. Rabbit:

Experiment No. 5. Report:

Experiment No. 6.

Subject's initials or No. -........................---------........................- Date....................------------------------------------.'. -

Age.................Sex................Height............... Weight.................... Medicine..................................................................................

Condition................................................................................................__ Dose................................-.................................................._

Thermometer............................................................................................ How administered........................................................__.

Barometer................................................................................................... Remarks..................................................................................

Relative humidity................................................................................... Reporter..................................................................................

Aconite 48

Experiment No. 7. Hospital report:

Experiment No. 8. Concerning the use of Aconite: Arguments pro and con: