1. Pith the brain of a frog. Obtain normal heart tracings; bathe heart with 20°/o Alcohol, and note results.

2. Obtain normal heart tracings from a pithed frog. Administer by stomach 1 mil 20% Alcohol. Continue tracings on both slow and fast drums.

3. Repeat Experiment No. 2, except to administer drug by thigh lymph sac.

4. In the anterior lymph sac of one frog inject 2 mils 25% Alcohol. Give the same dose by stomach to another frog of equal weight and condition. What results ? Observe again next day.

5. Having secured the normals of a rabbit, administer by stomach 5 mils x kg. 40% Alcohol, and note variations produced on all systems, including temperature. When the animal becomes stupefied, inject hypodermically 0.3 Gm. Caffeine.

6. Anaesthetize a cat or dog. Obtain the usual mechanical registrations of normals; also note temperature. Into the jugular vein, at the rate of 1 mil a minute, run 25% Alcohol, using warm Locke's solution for diluent, discontinuing the ether. Continue administration until animal dies. Endeavor to determine from your tracings at what systemic point the drug exerts its toxic effect. Compute amount of Alcohol used.

7. Select two subjects, one short and stout, the other tall and thin. Obtain normals of all the systems as usual. Test their reaction times to touch, hearing, and sight; test their mental activity with the antonym test, the imagery test, and the multiplication test; test strength with the grip dynamometer. Administer 20 mils French brandy, well diluted. Continue observations for two hours, taking the extra tests every half-hour. (This test is somewhat vitiated by the subject's anticipation. How might this be remedied?)

8. Since Alcohol has no advantageous utility in internal medicine, hospital observation will be available in the dipsomaniac wards only. Here study should be made of the pharmacodynamic reactions which may be registerable in both acute and chronic alcoholism.

9. Summarize and criticise three recent articles on the use of Alcohol in medicine, giving in each case your authority for position maintained.

Experiment No. 1. Heart tracings, with local application of drug. Frog:

Experiment No. 2. Heart tracings, with drug by stomach. Frog:

Experiment No. 3. Cardiac tracings, with drug by lymph sac. Frog:

Effect of drug by stomach and by lymph sac. Frogs:

Frog No. 1, stomach

Frog No. 2, lymph sac

Norm.

20'

40'

60'

Norm.

20'

40'

60'

Respiration

Swimm. reflex

Gen'l. reflex

Activity

Appearance next day

Experiment No. 5. Effect on rabbit of internal administration of drug:

Experiment No. 6. Kymograph records of effect on:

Experiment No. 7. Report on two men: A, short man; B, tall man:

Normal

After 30'

After 60'

After 90'

After 2 hours

A

B

A

B

A

B

A

B

A

B

AEsthesia

React. Sight

Sound

Touch

Mens, Antonyms

Imagery

Multiplication

Dynamometer

Alcohol 53

Subject's initials or No....................................................--------- Date........________________________________

Age.................Sex______Height...............Weight..........____ Medicine .....................-----------------------------------

Condition..........................................................................._________ Dose___________________________________

Thermometer................................................................................. How administered................-------------------------

Barometer........................................................_____............................. Remarks.....................................................................--------

Relative humidity..........................................._______________ Reporter......................................----------------...............

Experiment No. 8. Hospital reports. Reports concerning acute alcoholism:

Respiration

Heart-rate

Blood-pressure

Hour of observation

Case

Age

3rd

6th

9th

12th

3rd

6th

9th

12th

3rd

6th

9th

12th

I

S

D

S

D

S

D

S

D

II

III

IV

V

Reports concerning chronic alcoholism:

Respiration

Heart-rate

Blood-pressure

Day of observation

Case

Age

2nd

4th

6th

8th

2nd

4th

6th

8th

2nd

4th

6th

8th

I

S

D

S

D

S

D

S

D

II

III

IV

-

-

V

Experiment No. 9. Summary and criticism of arguments anent Alcohol: