1. Determine the effects on respiration and the reflexes of a frog of 0.1 Gm. Antipyrin injected into the anterior lymph sac.

2. Determine effect produced by bathing a frog's heart with a 1% solution of Antipyrin.

3. Determine the effects of Antipyrin on all the systems of a rabbit when 0.5 Gm. x kg. are administered by mouth.

4. Etherize a cat or rabbit. Connect with the several recording apparatus; obtain the usual normals. With the jugular vein connect a burette containing a 10% solution Antipyrin in warm Ringer's. Take continuous records while the solution runs in at the rate of 1 mil every 2 minutes. Report your conclusions, with graphic supporting evidence.

5. Select a subject who is not anaemic, and obtain the usual normals, including hemoglobin test and spectroscopic haemic test. Administer 0.5 Gm. Antipyrin (or 0.75 Gm. Acetphenetidin). Continue taking the usual records. Two hours after the drug has been taken secure another sample of blood for both haemoglobin and spectroscopic tests. In your report include your spectroscopic drawings.

6. In the hospital, study circulation and temperature reactions of patients to whom have been administered coal-tar preparations.

7. What are the relative merits and demerits of the several coal-tar products used in medicine?

Experiment No. 1. Report of effects produced on frog:

Experiment No. 2. Kymograph record. Frog:

Antipyrin 49

Experiment No. 3. Report of effect of 0.5 Gm. per kg. on rabbit:

Experiment No. 4. Kymograph records intravenous injection. Cat:

Antipyrin 50Antipyrin 51

Experiment No. 5.

Subject's initials or No. • - ....................-........................................ Date.....................___________________________

Age.................Sex______Height______Weight.................... Medicine ................----------------................................ -

Condition............................................................................................___ Dose............................______________.....................__

Thermometer............................................................................................ How administered.............................................................

Barometer................................................................................................... Remarks.........................„.......................................................

Relative humidity................................................................................... Reporter..................................................................................

Experiment No. 6. Hospital reports:

Heart-rate

Blood-pressure

Temperature

Drug

Dose

Now

15'

30'

Now

15'

30'

Now

15'

30'

Case No. I

-

Case No. II

Case No. III

Case No. IV

Case No. V

Case I

Case II

Case III

Case IV

Case V

Illness

Day of illness

Use of drug

Haemoglob. A Haemoglob. P

Spectrum A

Spectrum P

Experiment No. 7.

Drug

Merits

Demerits