1. Determine what effect Cannabis has on the heart of a frog when the drug is administered by stomach in the dose of 0.3 mil of the Tincture.

2. Determine the effect on the reflexes of a frog when 0.3 mil Tincture Cannabis is administered by lymph sac.

3. Determine the effect of Cannabis on all the systems of a cat, dog, or rabbit, when the drug is administered by stomach in doses of 0.3 mil x kg.

4. Etherize a dog or large cat, and obtain usual registrations. Administer by stomach 2 mils x kg. of an assayed Tincture of Cannabis. Stop the ether. Continue records. Any pupil variations?

5. Let a subject volunteer. Obtain the usual normals, and take record of his reflexes, size of pupil, and sensations of pain and of tactual space-discrimination. Let him describe a small simple picture at which he has gazed intently for 5 seconds. Administer 1 mil Tincture Cannabis. Continue usual observations every 10 minutes. At 40-minute intervals test his powers of description. Test also the relative keenness of pain and touch.

Do not engage him in conversation, but if he wishes to talk, note character, conformity, and reasonableness of whatever he says. If he experiences subjective sensations, let him describe them. Keep a careful record of all phenomena, noting also the advent of drowsiness, the time and character of sleep (omit blood-pressures now), and the mental and physical condition of the subject on awakening.

6. Interesting divergencies from the preceding experiment may be discovered by having a subject smoke (and inhale the smoke) 0.065 Gm. powdered Cannabis.

7. In the hospital assignment compare results obtained in Experiment No. 5 with the efficiency of the drug, as well as its physiological actions, when it is used to quiet cases of hysteria and nervous excitement.

8. In the psychopathic ward study available manifestations of the "hashish" habit.

9. From recent literature summarize at least three articles discussing the use of Cannabis in medicine.

Experiment No. 1. Heart tracings from stomach administration. Frog;

Experiment No. 2. Report of effect on reflexes, drug by lymph sac. Frog:

Experiment No. 3. Stomach administration to a

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Experiment No. 4. Kymographic records of stomach administration to

General statement:

Experiment No. 5.

Normal

40'

80'

120'

160'

Cervico-pupil reflex

Light-pupil reflex

Plantar-tickling reflex

Size of pupil

Tactile discrimination

Pain sensation, gms.

Volubility

Drowsiness

Sleep

Length of sleep:______________________________________________________

Quality of sleep:______________________________________________________

Discussion of effect on loquacity:_________________________________________

Discussion of effect on mentality:________________________________________

___________________________________________________________________

Discussion of subjective sensations:______________________________________

___________________________________________________________________________________________________

__________________________________________________________________________________________________

__________________________________________________________________________________________________

General statement:__________________________________________________

_________________________________________________________________

_________________________________________________________________

_________________________________________________________________

Subject's initials or No................................................... --------- Date..................................................................--------------

Age.................Sex__....... Height.................Weight:................... Medicine .................................................................„........._

Condition................................................................................................_ Dose.................................... - ............................................__

Thermometer............................................................:..-........................... How administered.........................................................._.

Barometer.........................'.......................................................................... Remarks..................................................................................

Relative humidity................................................................................... Reporter..................................................................................

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Experiment No. 7. Report of findings at the hospital:

Experiment No. 8. Report of findings in the psychopathic ward:

Report from literature:

Experiment No. 9.

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Subject's initials or No.....................................................---------- Date...................._______________________________

Age.................Sex____.......Height.................Weight.................... Medicine .................._........._....._...........------.....----------

Condition ._.......................................................................................____ Dose___.........___......______..............................................-

Thermometer............................................................................._............. How administered........................................................__

Barometer......................................................................................______ Remarks.....................______............................................._

Relative humidity................................................................................._ Reporter.................................................................................

Subject's initials or No.--------------------------------------- Date___________________________________

Age_______Sex______Height______Weight________ Medicine------------------------------........................_.

Condition__.........................___________________________ Dose___________________________________

Thermometer................................___................_.........__________ How administered..............................------------------

Barometer.......___________________________________ Remarks__....................................„................................. -

Relative humidity..........................................................„....................... Reporter________________________________

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Subject's initials or No.--------------------------------------- Date......................___________________________

Age___.........Sex______Height______Weight.................... Medicine _..................................--------------------------

Condition......................................_________........................_____ Dose__________.....-------..... - ......................______

Thermometer..:..................................................._....................._............. How administered........................................................__

Barometer................................................................................................... Remarks..................................................................................

Relative humidity................................................................................... Reporter____........................................................................

172.

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