1. Into the thigh lymph sac of a pithed frog inject 0.5 mil standardized Tincture Digitalis, diluted with an equal amount of Ringer's solution. Expose the heart, and keep it under observation for an hour or more, if necessary. Note variations in rates of auricles and ventricle; size, strength, and duration of diastole as compared with systole; color changes, etc. Keep heart moistened with Ringer's.

2. Pith a frog and secure normal heart tracings with a slow-timed drum, with an occasional curve on the fast drum. Administer by thigh lymph sac 0.5 mil diluted Tincture Digitalis. Continue tracings with slow drum, with occasional typical heart curves on the fast drum, until heart ceases beating. Compare results with those obtained in preceding experiment.

3. Pith a large frog; expose the heart, and secure normal tracings. Adjust apparatus for perfusion in situ with Infusion Digitalis (freshly made, and with salts added to the equivalence of Ringer's solution). Continue tracings until heart ceases beating, noting all objective and graphic variations.

4. Select 2 medium cats of equivalent weight and condition. Record normal heart-rate and respiration. To one give 0.5 mil Tincture Digitalis (diluted) by stomach; to the other give 3 mils freshly made Infusion Digitalis. Take regular observations of these two for several hours.

5. Select a large cat or medium-sized dog; etherize, and obtain the usual mechanical records of respiration and circulation. Into the jugular vein, at the rate of 1 mil a minute, run freshly made Infusion Digitalis (salted to be equivalent to Ringer's solution, and kept at 39° C). Take continuous records until animal expires.

6. Take 2 subjects of approximately similar weight, condition, and temperament. Obtain normals of pulse-rate, pulse energy, volume wave (by sphygmogram), respiration, blood-pressure, and temperature. Also, by auscultation, study quality and rhythm of apex beat. Then to one administer by mouth 1 mil Tincture Digitalis; to the other, 8 mils of fresh Infusion Digitalis. Take regular records every 10 minutes for 3 hours; then every 6 hours, until circulatory condition returns to normal.

Report if there be any alteration in the degree of kidney activity.

7. In the hospital make as many studies as opportunity permits on sundry cases receiving Digitalis treatment, using all the methods indicated in preceding experiment.

Because of the cumulative effect of Digitalis, caution must be exercised in determining what may be the present "normal" for the patient under study.

8. Write a concise summary of your findings in the laboratory; state what divergencies you may have observed in the hospital; and check all these with references from recent literature.

Experiment No. I

N.

5'

10'

15'

20'

25'

30'

35'

40'

45'

50'

55'

60'

Auricle

Rate

Diastolic %

Systolic %

Ventricle

Rate

Diastolic %

Systolic %

Color changes:. Remarks:.................

Experiment No. 2. Heart tracings from injected Tincture Digitalis. Frog:

Experiment No. 3. Heart tracings from perfused Infusion Digitalis. Frog:

Experiment No. 4.

Sheet No.

Date

Pharmacodynamics of Tincture Digitalis Dose, 0.5 mil x kg. Pharmacodynamics of Infus. Digitalis Dose, 3 mils x kg.

Administration, Stomach Service

Subject, Cats Age Sex Weight Condition

Temperature, Humidity, Reporter

Experiment No I 41

Red curve. Cat receiving the Tincture Digitalis. Blue curve. Cat receiving the Infusion Digitalis.

Experiment No. 5. Kymograph records of Infusion Digitalis intravenously:

Experiment No I 42

Experiment No. 6.

Subject's initials or No. -..............-------........................................ Date..............______________________________

Age.................Sex................Height.................Weight.................... Medicine1............................................................................ -

Condition.................................................................................................... Dose.......................................................................................

Thermometer..........................................................................................„ How administered...........................................................

Barometer................................................................................................... Remarks.............................................................................

Relative humidity................................................................................... Reporter..................................................................................

Experiment No. 6. Student taking I mil Tinct. =A; another, Infus. 7 mils =B.

Experiment No I 43

Experiment No. 7

Illness ................................................................. Duration of .................................................. Condition.......................

Present

0.5 hour

1 hour

1.5 hour

2 hours

3 hours

Dose=

Respiration

Heart-rate

Blood-press. S.

Blood-press. D

Pulse volume

Apex rhythm

Apex quality

Sphygmogram

Electrocardiogram

Later history:.......................................................................................................

Duplicate this study as many times as possible.

Experiment No. 8

Laboratory

Hospital

Literature

Respiration

Heart-rate

Blood-press.

Pulse volume

Diuresis

Apex rhythm

Vol. wave