1. Expose the heart of a pithed frog; connect with heart lever, and obtain a normal tracing. Apply to the heart 2 minims Epinephrin solution, 1 to 1000, and continue tracings, noting whatever effect may be produced.

2. Repeat Experiment No. 1, except to inject 0.1 mil of the Epinephrin solution into the thigh lymph sac.

3. Obtain a normal heart tracing from a pithed frog. Administer by lymph sac 0.2 mil 0.01 sol. Atropine Sulphate. Obtain brief records every few minutes. After 1/2 hour, apply some Epinephrin solution, and observe resulting tracing. Compare it with that obtained in Experiment No. I.

4. Etherize a dog, and make connections with respiratory and circulatory recording apparatus. To one carotid attach a mercury manometer; to the other attach a Hurthle manometer, and adjust it for marked upward excursion. Secure normals, then, with continuous drum, with hypodermic needle inject into jugular vein 0.05 mil per kg. Epinephrin solution, 1 to 1000, marking drum at moment of injection.

When normal conditions return, repeat injection, comparing time and extent of response.

When normal conditions return, readjust the Hurthle manometer for downward excursions. Administer via jugular vein with hypodermic 0.5 mil per kg. of Nitroglycerin, 1 to 1000 solution. When blood-pressure is at its lowest point, inject 0.05 mil per kg. Epinephrin solution, 1 to 1000.

When normal conditions return, reverse the preceding experiment.

Cause death with Epinephrin solution, noting phenomena.

5. Select a group of three students of equivalent weight, temperament, and condition. Record their normals; then administer 0.5 mil Epinephrin solution, 1 to 1000 - to one by mouth, to a second subcutaneously, to a third intramuscularly. Take note of all reactions. Compare with others. See particularly if reaction seems to be modified in any way by temperament or physique.

6. Study the uses of Epinephrin in the operative clinics of ophthalmology and rhinology.

Ascertain also in what ward, and for what conditions, the further utility of Epinephrin is being studied. Learn the present status of such investigations, and compare results with what you have thus far obtained.

7. Marshal in systematic form the leading facts known about the pharmacodynamics of Epinephrin, giving your authority in each case.

Experiments No. I, 2 And 3. Kymograph Records. Frog

Experiment No. 4. Kymograph records. Dog:

Experiment No. 5.

Subject's initials or No..............-----------------...............------ Date....................-----------------------------------------

Age.................Sex................Height.................Weight.................... Medicine ..........................................................................----

Condition..........................................................................................------ Dose....................-----------------...................................._

Thermometer............................................................................................ How administered....................................................-----

Barometer................................................................................................... Remarks...............................................................................„

Relative humidity................................................................................... Reporter..................................................................................

Experiments No I 2 And 3 Kymograph Records Frog 44

Experiment No. 5.

By mouth

Subcutaneously

Intramuscularly

Subject 1; wt.

Subject 2; wt.

Subject 3; wt.

age, ; sex,

age, ; sex,

age, ; sex,

temperament,

temperament,

temperament,

Max.0

Time'

Norm.'

Max.o

Time'

Norm.'

Max.0

Time '

Norm.'

Pulse

Respiration

Blood-press. S.

Blood-press. D.

.

Pupil

Subject 4; wt.

Subject 5; wt.

Subject 6; wt.

age, ; sex,

age, ; sex,

age. ; sex,

temperament,

temperament,

temperament,

Max.o

Time'

Norm.'

Max.o

Time '

Norm.'

Max.o

Time '

Norm.'

Pulse

Respiration

Blood-press. S.

Blood-press. D.

Pupil

Subject 7; wt.

Subject 8; wt.

Subject 9; wt.

age, ; sex,

age, ; sex,

age, ; sex,

temperament,

temperament,

temperament,

Max.°

Time'

Norm.'

Max.o

Time '

Norm.'

Max.o

Time '

Norm.'

Pulse

Respiration

Blood-press. S.

Blood-press. D.

Pupil

Experiment No. 6. Report of observations in operative ophthalmology:

Report of observations in operative rhinology:

Report of other observations in the hospital:

Experiment No. 7. Summary of pharmacodynamics of Epinephrin: