1. Into the anterior lymph sac of a male frog inject I mil of a 4% solution Morphine Sulphate. Observe reactions for 2 hours in comparison with a normal frog of equal weight and with another frog of same weight which has had a like dose 2 hours previously. Note how these animals respond to sundry stimuli akin to that experienced in their normal environment.

2. Take 2 frogs of equal weight and condition. Compare their reactions when one has 1 mil of a 4% solution Morphine Sulphate injected into its stomach, while the other has a like dose injected into its anterior lymph sac.

3. Pith each brain of 2 frogs of equal weight and condition. By leg lymph sac administer to one 1 mil of a 4% solution Morphine Sulphate, to the other a like dose, but containing in addition 0.002 Gm. Atropine Sulphate. Arrange for heart tracings, and secure intermittent comparative records for the next two hours. Frogs must be kept moist, and at a constant cool temperature. What do your graphs seem to indicate?

4. Take a cat, a rabbit, and a small dog. Obtain an average of 3 observations on each of heart-rate and respiration. To the rabbit give hypodermically of Morphine Sulphate 0.020 Gm. per kilogram body-weight; to the cat the same way, 0.050 Gm. per kilo; to the dog the same way, 0.020 Gm. per kilo. Carefully observe the comparative sequence of phenomena elicited by Morphine Sulphate on these types of mammals.

5. When the animals in Experiment No. 4 show evidences of narcotism, lightly etherize them, place on animal-board, connect with recording apparatus. Remove the ether. Continue records until death ensues, administering more of the Morphine if necessary. (Sollmann gives the minimal fatal dose (M. F. D.) of Morphine as follows: dog, 0.4 Gm. x kg.; cat, 0.04 to 0.08 Gm. x kg.; rabbit, 0.3 Gm. x kg.)

6. Select 3 fasting rabbits of like weight and condition. Administer by stomach, to one, Morphine Sulphate 0.020 Gm. x kg.; to the second, Tincture Opium, 2 mils x kg.; to the third, Morphine Sulphate, 0.020 Gm. and Atropine Sulphate 0.002 Gm. x kg. Compare reactions throughout laboratory period.

7. Let a member of the class volunteer to take 0.020 Gm. Morphine Sulphate. Let him be seated in a comfortable position, in a steamer chair for example. Secure his normal pulse, respiration, blood-pressure and temperature. With aesthesiometer test his forearm and forehead for tactile discrimination. Test his reaction time for sight, hearing, feeling. Make speed tests of his mental activity, using the antonym test, the test for imagery, and the multiplication test. Let him take the drug in solution. Take systemic observations every 10 minutes, and mental and aesthesic tests every half hour.

8. In the hospital, study various cases to whom Morphine has been administered by the attending physician.

9. In the psychopathic ward, study cases under treatment for the relief or cure of morphinism.

10. Consult recent literature for at least four discussions of the pharmacology of Morphine. Write up a brief resume of your laboratory findings, checking the same with citations from your readings, either confirmative or otherwise.

## Experiments With Morphine. Experiment No. I

 Observation Normal frog Narcotized frog Narcotized frog 2 Excitability ; 30 min. 60 min. 90 min. 30 min. 60 min. 90 min. 30 min. 60 min. 90 min. Climbing incline Swimming Escaping from under submerged beaker Cutan. sensibility Clasping female

Respiration grapbs: 0 5 10 15 20 25 303540 455055 60 5 10 15 20 25 30 35 40 45 50 55 60

## Experiment No. 3

Paste in samples of your Kymograph records.

## Experiments No. 4 And 5. Graphs Of Respiration And Pulse*

 Observation Rabbit Time Cat Time Dog Time Disposition Alterations of D. Restlessness Excitement Frenzy Salivation Nausea Vomiting Defecation Drowsiness Stupor

* For uniformity use red ink for cat, blue for dog, and green for rabbit.

## Experiments No. 4 And 5 (Continued.)

 Pupil changes Elapsed Time in Minutes 10 20 30 40 50 60 10 20 30 40 50 Rabbit Cat Dog
 Rabbit Cat Dog Cause of death Time elapsed

Kymograph records:

## Experiment No. 6

 Rabbit No. 1 Morphine Rabbit No. 2 Tincture Opium Rabbit No. 3 Morph. + Atrop. Mental variations Excitement Stupor Lethargy Pupil changes
 Normal After 30 ' After 60 ' After 90 ' After 2 hours AEsthesia Reaction time Sight Hearing Touch Mental agility Antonyms Imagery Multiplication

## Experiment No. 7

Subject's initials or No..................................................................................................................... Date..........................................................................................

Age............................Se...........x................Height............................Weight............................... Medicine................................................................................

Condition................................................................................................................................................ Dose..........................................................................................

Barometer................................................................................................... Remarks..................................................................................

Relative humidity................................................................................... Reporter..................................................................................

## Experiment No. 8

Sex of patient,.........................................; approximate age,........................; illness, ............................................

date of illness,............................................; present condition of patient,...........................................................................