1. Into the anterior lymph sac of a male frog inject I mil of a 4% solution Morphine Sulphate. Observe reactions for 2 hours in comparison with a normal frog of equal weight and with another frog of same weight which has had a like dose 2 hours previously. Note how these animals respond to sundry stimuli akin to that experienced in their normal environment.

2. Take 2 frogs of equal weight and condition. Compare their reactions when one has 1 mil of a 4% solution Morphine Sulphate injected into its stomach, while the other has a like dose injected into its anterior lymph sac.

3. Pith each brain of 2 frogs of equal weight and condition. By leg lymph sac administer to one 1 mil of a 4% solution Morphine Sulphate, to the other a like dose, but containing in addition 0.002 Gm. Atropine Sulphate. Arrange for heart tracings, and secure intermittent comparative records for the next two hours. Frogs must be kept moist, and at a constant cool temperature. What do your graphs seem to indicate?

4. Take a cat, a rabbit, and a small dog. Obtain an average of 3 observations on each of heart-rate and respiration. To the rabbit give hypodermically of Morphine Sulphate 0.020 Gm. per kilogram body-weight; to the cat the same way, 0.050 Gm. per kilo; to the dog the same way, 0.020 Gm. per kilo. Carefully observe the comparative sequence of phenomena elicited by Morphine Sulphate on these types of mammals.

5. When the animals in Experiment No. 4 show evidences of narcotism, lightly etherize them, place on animal-board, connect with recording apparatus. Remove the ether. Continue records until death ensues, administering more of the Morphine if necessary. (Sollmann gives the minimal fatal dose (M. F. D.) of Morphine as follows: dog, 0.4 Gm. x kg.; cat, 0.04 to 0.08 Gm. x kg.; rabbit, 0.3 Gm. x kg.)

6. Select 3 fasting rabbits of like weight and condition. Administer by stomach, to one, Morphine Sulphate 0.020 Gm. x kg.; to the second, Tincture Opium, 2 mils x kg.; to the third, Morphine Sulphate, 0.020 Gm. and Atropine Sulphate 0.002 Gm. x kg. Compare reactions throughout laboratory period.

7. Let a member of the class volunteer to take 0.020 Gm. Morphine Sulphate. Let him be seated in a comfortable position, in a steamer chair for example. Secure his normal pulse, respiration, blood-pressure and temperature. With aesthesiometer test his forearm and forehead for tactile discrimination. Test his reaction time for sight, hearing, feeling. Make speed tests of his mental activity, using the antonym test, the test for imagery, and the multiplication test. Let him take the drug in solution. Take systemic observations every 10 minutes, and mental and aesthesic tests every half hour.

8. In the hospital, study various cases to whom Morphine has been administered by the attending physician.

9. In the psychopathic ward, study cases under treatment for the relief or cure of morphinism.

10. Consult recent literature for at least four discussions of the pharmacology of Morphine. Write up a brief resume of your laboratory findings, checking the same with citations from your readings, either confirmative or otherwise.

Experiments With Morphine. Experiment No. I

Observation

Normal frog

Narcotized frog

Narcotized frog 2

Excitability

; 30 min.

60 min.

90 min.

30 min.

60 min.

90 min.

30 min.

60 min.

90 min.

Climbing incline

Swimming

Escaping from under submerged beaker

Cutan. sensibility

Clasping female

Respiration grapbs: 0 5 10 15 20 25 303540 455055 60 5 10 15 20 25 30 35 40 45 50 55 60

Experiment No. 2

Briefly state your findings.

Experiment No. 3

Paste in samples of your Kymograph records.

What are your deductions?

Experiment No 3 27

Experiments No. 4 And 5. Graphs Of Respiration And Pulse*

Experiments No 4 And 5 Graphs Of Respiration And P 28

Observation

Rabbit

Time

Cat

Time

Dog

Time

Disposition

Alterations of D.

Restlessness

Excitement

Frenzy

Salivation

Nausea

Vomiting

Defecation

Drowsiness

Stupor

* For uniformity use red ink for cat, blue for dog, and green for rabbit.

Experiments No. 4 And 5 (Continued.)

Pupil changes

Elapsed Time in Minutes

10

20

30

40

50

60

10

20

30

40

50

Rabbit

Cat

Dog

Rabbit

Cat

Dog

Cause of death

Time elapsed

Kymograph records:

Experiment No. 6

Rabbit No. 1 Morphine

Rabbit No. 2 Tincture Opium

Rabbit No. 3 Morph. + Atrop.

Mental variations

Excitement

Stupor

Lethargy

Pupil changes

Experiment No 6 29

Normal

After 30 '

After 60 '

After 90 '

After 2 hours

AEsthesia

Reaction time Sight

Hearing

Touch

Mental agility Antonyms

Imagery

Multiplication

Experiment No. 7

Subject's initials or No..................................................................................................................... Date..........................................................................................

Age............................Se...........x................Height............................Weight............................... Medicine................................................................................

Condition................................................................................................................................................ Dose..........................................................................................

Thermometer............................................................................................ How administered............................................................

Barometer................................................................................................... Remarks..................................................................................

Relative humidity................................................................................... Reporter..................................................................................

Experiment No 7 30

Experiment No. 8

Sex of patient,.........................................; approximate age,........................; illness, ............................................

date of illness,............................................; present condition of patient,...........................................................................