1. Obtain a heart tracing from a frog with pithed brain. Apply 2 drops 0.6% solution Pilocarpine to the frog's heart, carefully noting any variations in the record. Wash the heart with normal saline (=0.65% for frogs), and then apply 2 drops 0.1% solution Atropine Sulphate. When definite effect is produced, wash again with normal saline; then study effect of alternation of the drugs, noting relative amounts of each required to produce definite results. Allow at least 30 seconds between successive applications.

2. Repeat Experiment No. 1, using 0.03% Physostigmine solution in place of the Pilocarpine.

3. Having obtained a heart tracing of a frog with pithed brain, inject into the thigh lymph sac 0.5 mil of a 0.6% solution Pilocarpine, and note what effect this may have on the tracing. See also if 1 drop of a 0.01% solution Atropine Sulphate will produce a distinct variation in the curve.

4. Repeat Experiment No. 3, using 0.03% solution Physostigmine.

5. Into thigh lymph sacs of two frogs of equal weight and condition inject 0.3 mil of a 0.01% solution Atropine Sulphate. Obtain continuous tracings, and when the Atropine action is marked, see how much Pilocarpine solution, 0.6%, applied locally at the rate of 2 drops every 30 seconds, is necessary to overcome the Atropine action in one frog, and how much Physostigmine in the same ratio of increments is necessary with the other frog.

6. Into one eye of a rabbit instill 1 drop 0.6% solution Pilocarpine; repeat with another rabbit, using 0.03% solution Physostigmine. Study the effect for one-half hour; then instill in the treated eye of each rabbit 1 drop of 0.01% Atropine solution. Seek an explanation.

7. Etherize a rabbit, and secure normal respiratory and circulatory tracings. Rapidly dissect the skin from the abdomen, and notice coils of intestine showing through the thin muscle walls. With etherization light, administer by vein 3 mils x kg. of a 1 to 1000 solution Pilocarpine, obtaining meanwhile a continuous record, and noting all phenomena. When reaction becomes pronounced, administer by vein 1.5 mg. x kg. Atropine in solution.

Later, cause death with 10% Pilocarpine, taking continuous record.

8. Repeat Experiment No. 8, using Physostigmine, 1 mg. x kg.

9. Let some student, who is normally somewhat constipated, take 1 mg. Physostigmine (Eserine) Salicylate. Let his associates take the customary systemic records.

10. In the ophthalmology clinic, study the use there of both Pilocarpine and Physostigmine.

11. In the hospital assignment, an opportunity may arise, in connection with postoperative observations in abdominal surgery, to study the action of Physostigmine; and in the medical ward to study the action of Pilocarpine in the treatment of uraemia.

12. Tabulate your findings concerning these two drugs. Extend your tabulations by observations drawn from recent literature.

Experiment No. I. Kymograph Records. Pilocarpine, 0.6%; Atropine, 0.1%

Normal

Pilocarp.

2 gtt.

NaCl

Atropine,

2 gtt.

"Normal," No. 2

2nd Piloc.

gtt.

NaCl, No. 2

2nd Atrop.

gtt.

"Normal," No. 3

3rd Piloc.

gtt.

NaCl, No. 3

3rd Atrop.

gtt.

Experiment No. 2. Kymograph Records. Physostigmine, 0.03%; Atropine, 0.1%

Normal

Physost.

2 gtt.

NaCl

Atropine,

2 gtt.

"Normal," No. 2

2nd Physost

gtt.

2nd NaCl

2nd Atrop.

gtt.

"Normal," No. 3

3rd Physost.

gtt.

3rd NaCl

3rd Atrop.

gtt.

Experiment No. 3

Normal

Pilocarpine, 0.6%

Atropine, 0.01%

Experiment No. 4. Kymograph Records

Normal

Physostigmine, 0.03%

Atropine, 0.01%

Experiment No. 5

Atropine,

0.3 mg.

Pilocarpine,

mg.

Atropine,

0.3 mg.

Physostig.,

mg.

Experiment No. 6

N

10'

20'

30'

Atropine

5'

10'

15'

20'

Pilocarpine

Physostigmine

Experiment No. 7

Respiration

Circulation

Normal

Pilocarpine

Atropine

F. D. Pilocarpine

Experiment No. 8. Kymograph Records

Respiration

Circulation

Normal

Physostigmine

Atropine

F. D. Physostig.

Experiment No. 9

Report of internal symptoms experienced by subject:................................................................................

................................................._...............................

................................................._...............................

Experiment No. 10

Why used

Amount

Interval

Result

Findings

Pilocarpine

2nd Case

3rd Case

Physostigmine

2nd Case

3rd Case

Subject's initials or No..................................................................... Date......................................................................................._.

Age...............;.Sex................Height.................Weight.................... Medicine..................................................................................

Condition.................................................................................................... Dose.....................................................,.................................._

Thermometer............................................................................................ How administered.............................................................

Barometer................................................................................................... Remarks..................................................................................

Relative humidity................................................................................... Reporter........................................_.......___.......................

Experiment No 10 35

Experiment No. 11

Why ordered

Resp.

Pulse

B. P.

Interval

Result

Resp.

Pulse

B. P.

Pupil

Pilocarpine

2nd Case

3rd Case

Physostigmine

2nd Case

3rd Case

Experiment No. 12. Your Experience, Checked By References

Pulse

Resp.

B.P.

Eye

Saliv. gland

Other glands

Alim. tract

Bronchi

Addenda

Pilocarpine

Ref. 1=

Ref. 2=

Ref. 3=

Physostigmine

Ref. 1=

Ref. 2=

Ref. 3=