1. Obtain a heart tracing from a frog with pithed brain. Apply 2 drops 0.6% solution Pilocarpine to the frog's heart, carefully noting any variations in the record. Wash the heart with normal saline (=0.65% for frogs), and then apply 2 drops 0.1% solution Atropine Sulphate. When definite effect is produced, wash again with normal saline; then study effect of alternation of the drugs, noting relative amounts of each required to produce definite results. Allow at least 30 seconds between successive applications.

2. Repeat Experiment No. 1, using 0.03% Physostigmine solution in place of the Pilocarpine.

3. Having obtained a heart tracing of a frog with pithed brain, inject into the thigh lymph sac 0.5 mil of a 0.6% solution Pilocarpine, and note what effect this may have on the tracing. See also if 1 drop of a 0.01% solution Atropine Sulphate will produce a distinct variation in the curve.

4. Repeat Experiment No. 3, using 0.03% solution Physostigmine.

5. Into thigh lymph sacs of two frogs of equal weight and condition inject 0.3 mil of a 0.01% solution Atropine Sulphate. Obtain continuous tracings, and when the Atropine action is marked, see how much Pilocarpine solution, 0.6%, applied locally at the rate of 2 drops every 30 seconds, is necessary to overcome the Atropine action in one frog, and how much Physostigmine in the same ratio of increments is necessary with the other frog.

6. Into one eye of a rabbit instill 1 drop 0.6% solution Pilocarpine; repeat with another rabbit, using 0.03% solution Physostigmine. Study the effect for one-half hour; then instill in the treated eye of each rabbit 1 drop of 0.01% Atropine solution. Seek an explanation.

7. Etherize a rabbit, and secure normal respiratory and circulatory tracings. Rapidly dissect the skin from the abdomen, and notice coils of intestine showing through the thin muscle walls. With etherization light, administer by vein 3 mils x kg. of a 1 to 1000 solution Pilocarpine, obtaining meanwhile a continuous record, and noting all phenomena. When reaction becomes pronounced, administer by vein 1.5 mg. x kg. Atropine in solution.

Later, cause death with 10% Pilocarpine, taking continuous record.

8. Repeat Experiment No. 8, using Physostigmine, 1 mg. x kg.

9. Let some student, who is normally somewhat constipated, take 1 mg. Physostigmine (Eserine) Salicylate. Let his associates take the customary systemic records.

10. In the ophthalmology clinic, study the use there of both Pilocarpine and Physostigmine.

11. In the hospital assignment, an opportunity may arise, in connection with postoperative observations in abdominal surgery, to study the action of Physostigmine; and in the medical ward to study the action of Pilocarpine in the treatment of uraemia.

12. Tabulate your findings concerning these two drugs. Extend your tabulations by observations drawn from recent literature.

Experiment No. I. Kymograph Records. Pilocarpine, 0.6%; Atropine, 0.1%

Experiment No. 2. Kymograph Records. Physostigmine, 0.03%; Atropine, 0.1%

Experiment No. 3

Experiment No. 4. Kymograph Records

Experiment No. 5

Experiment No. 6

Experiment No. 7

Experiment No. 8. Kymograph Records

Experiment No. 9

Report of internal symptoms experienced by subject:................................................................................

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Experiment No. 10

Subject's initials or No..................................................................... Date......................................................................................._.

Age...............;.Sex................Height.................Weight.................... Medicine..................................................................................

Condition.................................................................................................... Dose.....................................................,.................................._

Thermometer............................................................................................ How administered.............................................................

Barometer................................................................................................... Remarks..................................................................................

Relative humidity................................................................................... Reporter........................................_.......___.......................

Experiment No. 11

Experiment No. 12. Your Experience, Checked By References