To follow these without the loss of an instant's vision was pleasure of the highest kind. In an hour and ten minutes from their first discovery they had grown to oval points. In one hour more the specks had become beaked and long. And this pointed end was universally the end from which the flagellum emerged. With the flagellum comes motion, and with that abundant pabulum, and therefore rapid growth. But when motion is attained we are compelled to abandon the mass and follow one in all its impetuous travels in its little world; and by doing so we are enabled to follow the developed speck into the parent condition and size, and not to leave it until it had, like its predecessors, entered on and completed its wonderful self-division by fission.

It becomes then clearly manifest that these organisms, lowly and little as they are, arise in fertilized parental products. There is no more caprice in their mode of origin than in that of a crustacean or a bird. Their minuteness, enormous abundance, and universal distribution is the explanation of their rapid and practically ubiquitous appearance in a germinating and adult condition. The presence of putrefiable or putrescent matter determines at once the germination of the always-present spore. But a new question arises. These spores are definite products. In the face of some experimental facts one was tempted to inquire: Have these spores any capacity to resist heat greater than the adults? It was not easy to determine this question. But we at length were enabled to isolate the germs of seven separate forms, and by means of delicate apparatus, and some twelve months of research, to place each spore sac in an apparatus so constructed that it could be raised to successive temperatures, and without any change of conditions examined on the stage of the microscope.

In this way we reached successive temperatures higher and higher until the death point - the point beyond which no subsequent germination ever occurred - was reached in regard to each organism. The result was striking. The normal death point for the adult was 140° F. One of the monads emitted from its sac minute mobile specks - evidently living bodies - which rapidly grew. These we always destroyed at a temperature of 180° F. Three of the sacs emitted spores that germinated at every temperature under 250° F. Two more only had their power of germination destroyed at 260° F. And one, the least of all the monad forms, in a heat partially fluid and partially dry, at all points up to 300° F. But if wholly in fluid it was destroyed at the point of 290° F. The average being that the power of heat resistance in the spore was to that of the adult as 11 to 6. From this it is clear that we dare not infer spontaneous generation after heat until we know the life-history of the organism.

In proof of this I close with a practical case. A trenchant and resolute advocate of the origin of living forms de novo has published what he considers a crucial illustration in support of his case. He took a strong infusion of common cress, placed it in a flask, boiled it, and, while boiling, hermetically sealed it. He then heated it up in a digester to 270° F. It was kept for nine weeks and then opened, and, in his own language, on microscopical examination of the earliest drop "there appeared more than a dozen very active monads." He has fortunately measured and roughly drawn these. A facsimile of his drawing is here. He says that they were possessed of a rapidly moving lash, and that there were other forms without tails, which he assumed were developmental stages of the form. This is nothing less than the monad whose life-history I gave you last. My drawings, magnified 2,500 diams., of the active organism and the developing sac are here.

Now this experimenter says that he took these monads and heated them to a temperature of about 140° F., and they were all absolutely killed. This is accurately our experience. But he says these monads arose in a closed flask, the fluid of which had been heated up to 270° F. Therefore, since they are killed at 140° F., and arose in a fluid after being heated to 270° F., they must have arisen de novo! But the truth is that this is the monad whose spore only loses its power to germinate at a temperature (in fluid) of 290°, that is to say, 20° F. higher than the heat to which, in this experiment, they had been subjected. And therefore the facts compel the deduction that these monads in the cress arose, not by a change of dead matter into living, but that they germinated naturally from the parental spore which the heat employed had been incompetent to injure. Then we conclude with a definite issue, viz., by experiment it is established that living forms do not now arise in dead matter. And by study of the forms themselves it is proved that, like all the more complex forms above them, they arise in parental products.

The law is as ever, only that which is living can give origin to that which lives.

[3]A magnified image of the bee's sting was projected on the screen.[4]A series of the eggs of butterflies were then shown, as were the objects successively referred to, but not here reproduced.[5]The pygidium of the flea, very highly magnified, was here shown.[6]An illustration of the pygidium structure seen with one-thirty-fifth immersion was given.