The routine of this later experiment was but little modified from that of the experiment of November 21 to 23, the records of the body activity being substantially the same as in the earlier experiment. The body-weight without clothing at 7 o'clock each morning was 65.8 kilograms, 64.6 kilograms, and 63.4 kilograms for the 3 days, respectively, indicating the usual somewhat rapid loss in weight during the first few days of fasting. The records of the pulse rate, respiration rate, strength tests, and body-temperature did not vary appreciably from the values obtained in the earlier experiments with this subject and with others. On the first fasting day the subject drank 114.7 grams of water and the weight of urine was 526.8 grams; on the second day he drank 186.3 grams of water and the weight of urine was 569.1 grams. The carbon-dioxide production, oxygen consumption, and water vaporization were determined as usual in 2-hour periods throughout the entire experiment; the nitrogen in the urine was also determined. From these values the complete metabolism was obtained. The data for the total carbon-dioxide production, oxygen consumption, and heat production, are given in table 14 (see p. 64); those obtained from the analysis of the urine are given in table 15. The subdivision of the income and outgo in terms of elements is shown in table 16, while the elements and materials katabolized, which have been computed in accordance with the usual method,3 are recorded in table 17.

1Benedict, Carnegie Inst. Wash. Pub. No. 77, 1907, p. 222 et seq. 2See, also, statistics for the fasting days Dec. 3 to 5, 1906, on p. 251. 3Benedict, Carnegie Inst. Wash. Pub. No. 77, 1907, p. 37.

A comparison of the energy as computed from body material katab-olized with the heat production as measured is given in table 18.

Table 14. - A. H. M"; December 3-6, 1906. (24-Hour Periods, 7 A. M. To 7 A. M.) Mayonnaise, Lettuce, And Lemon

Amount, 213 grams; nitrogen, 0.37 gram; energy, 1,112 calories.

Fuel value: Total, 1,109 calories; from protein, 1 percent; from fat, 98 per cent; from carbohydrates, 1 per cent.

1Subject finished eating about 2\ hours after beginning of period.

Table 15. - Weight, Composition, And Heat Of Combustion Of Urine In Fasting Experiment With A. H. M., December 8-5, 1906

1In terms of creatinine.

The carbon-dioxide production and oxygen consumption for the two fasting days agree very closely, but there is a difference of approximately 120 calories in the heat production. The respiratory quotient for the first day of fast was 0.81 and for the second 0.77. These values are somewhat higher than the average values found for all the subjects of short fasts recorded in the earlier report,1 which were for the first day of fast 0.79 and for the second 0.75, although in at least two instances in these short fasts a value was found as high as 0.77 on the second day.

1Benedict, Carnegie Inst. Wash. Pub. No. 77, 1907, p. 451, table 225.

Table 16. - Elements Katabolized In Body In Fasting Experiment With A. H. M., December S-5, 1906

1Weight of urine less solid matter. Solid matter for Dec. 3-4 calculated from nitrogen by using ratio solid matter/N for Dec. 4-5, 1906. (See table 15).

2Sum of nitrogen, carbon, hydrogen, and oxygen.

3N X0.829 (see average ratio C/N, Benedict, Carnegie Inst. Wash. Pub. No. 77, 1907, table 202. p. 384).

4N X 0.230 (computed ratio H/N for experiment No. 81; see Benedict, Carnegie Inst. Wash.

Pub. No. 77, 1907, table 168, pp. 258 and 259).

5N X 0.844 (computed ratio O/N for experiment No. 81; see Benedict, Carnegie Inst. Wash.

Pub. No. 77, 1907, table 168, pp. 258 and 259).

Table 17. - Elements And Materials Katabolized In Body In Fasting Experiment With A.H. M. December S-5, 1906.1

1See table 16 for methods of obtaining data.

Table 18. - Comparison Of Energy Computed From Body Material Katabolized, With Heat Production As Measured In Fasting Experiment With A. H. M., December 3-5, 1906

A reference to tables 15 and 16 shows that there was the usual rise in the nitrogen excretion on the second day of fasting. Of particular interest is the fact that the amount of glycogen katabolized, as shown in table 17, was 123.1 grams on the first day and 47.6 grams on the second day, this agreeing very well with the averages found for the other fasting subjects, namely, 110 grams on the first day and 40.3 grams on the second day.1

It is thus clear that the tendency to store glycogen shown in the experiment of November 21 to 23,1905, was not at all characteristic of this subject.2 This fact is further emphasized by the data obtained in the 1-day food experiment with an exclusively fat diet which followed the 2 days of fast, these results showing a further katabolism of glycogen amounting to 47.3 grams. Apparently the subject had by no means exhausted his glycogen supply at the end of the 2 days of fasting, even with a total output of 170.7 grams for the 2 fasting days.

Although it is contended that substances other than creatinine affect the Jaffe color reaction and accordingly the determinations of creatine in fasting urine can not be absolutely relied upon,3 it should be noted that in this experiment, as in the earlier fasting experiments, there was evidence of preformed creatine in the urine. As a matter of fact, the 0.02 gram of creatine excreted on the second day of fast (see table 15) is much less than was observed in any of the other fasting experiments, the tendency in the earlier experiments being for this factor to increase somewhat on the second day rather than to decrease as in this experiment.1 Inasmuch as so long a time has elapsed since the fasting experiment in December 1906 was made, and particularly as full reports of both short and long fasts have been given in recent publications,2 it seems unnecessary to discuss in further detail the results of this experiment.

1Benedict, Carnegie Inst. Wash. Pub. No. 77, 1907, p. 464, table 228.

2Ibid., p. 222 et seq.

3Graham and Poulton, Proc. Royal Soc, ser. B, 1914, 87, p. 205.