The addition of fat or sugar to meat diet allows of a considerable reduction in the supply of meat, both the body weight and nitrogenous tissue change preserving their equilibrium on a smaller amount of food. It has been estimated that the nitrogenous tissue change is reduced seven per cent, by the addition of fat, and ten per cent, by the addition of carbohydrate food to the meat diet; therefore less meat is wanted to make up nitrogenous tissues. Further, fats and sugars, which obviously cannot of themselves form an adequate diet, since they contain no nitrogen, seem to have the power of accomplishing some end in the economy which, in their absence, requires a considerable expenditure of nitrogenous materials to bring about. Fats and sugars, then, supply to the body readily oxidizable materials, and thus shield the albuminous tissues from oxidation, as well as reduce absolutely the nitrogenous metabolism.

It would further appear from the experience gained from the stall feeding of animals that a good supply of carbohydrates, together with a limited quantity of nitrogenous food, is admirably adapted to produce fat. Since much more fat has been found to be produced in pigs than could be accounted for by the albuminous and fatty constituents of their diet, we must suppose that from their carbohydrate food fat can be manufactured in their body.

Much of the difficulty found in reconciling the opinions of different authors concerning the sources of fat in the body can be removed, and some knowledge of the manufacture of fats from the food stuffs can be gained by bearing in mind the properties of the protoplasm. There can be no doubt that protoplasm, if properly nourished, can manufacture fat. As examples, we may take the cells of the mammary gland and connective tissue. This fat production may be regarded as a secretion of fat, though only in one of the examples given does it appear externally as a definite secretion - milk. We cannot scrutinize the chemical methods by which this change is brought about in protoplasm, any more than those which give rise to the special constituents of other secretions. We know that protoplasm uses as pabulum, albumin, fat, and carbohydrate, and we have no reason to doubt that the proportion of these materials found to form the most nutritious diet for the body generally, is also the proportion in which protoplasm can best make use of them. Probably cells which secrete a material containing nitrogen, such as mucin-yielding gland cells, require a greater proportion of albumin. Those cells which produce a large quantity of non-nitrogenous material may not require more nitrogen than is necessary for their perfect re-integration as nitrogenous bodies. In the manufacture of their secretion, they only require a pabulum which contains the same chemical elements as are to be found in the output. In the case of fat formation a supply of fat or carbohydrate ought to suffice if accompanied by a small amount of albuminous substance. If these non-nitrogenous substances be withheld, the protoplasm could no doubt obtain the quantity of carbon, hydrogen, and oxygen requisite to manufacture fat from albumin, but this would not be economical, for a large amount of nitrogen would be wasted.

Fat cannot be produced by the tissue cells without nitrogen in the diet, because the fat-manufacturing protoplasm cannot live without nitrogen, which is absolutely necessary for its own assimilative re-integration. A good supply of nitrogenous food aids in fattening, since it gives vigor to all the protoplasmic metabolism, and among them fat formation.

The albuminoid substance gelatine, which is an important item in the food we ordinarily make use of, is able to effect a saving in the albuminous food stuffs. Although it contains a sufficiently large proportion of nitrogen, it cannot satisfactorily replace albumin in the food. Indeed, in spite of the great similarity in its chemical composition to albuminous bodies, it can no better replace the proteids in a dietary than fat or carbohydrate; and, although an animal uses up less of its tissue nitrogen on a diet containing gelatine and fat than when it is fed on fat alone, it dies of starvation almost as soon as if its diet contained no nitrogenous substance.