"I made several experiments, to see if there was such a quantity of oil in it, as would account for the quantity of wax to be formed, and to learn if it was composed of oil. I held it near the candle; it burnt, but did not smell like wax, and had the same smell when burning, as farina when it was burnt. I observed, that this substance was of different colours on different bees, but always of the same colour on both legs of the same bee; whereas a new-made comb was all of one co lour. I observed, that it was gathered with more avidity for old hives, where the comb is complete, than for those hives where it was only begun, which we could hardly conceive, admitting it to be the materials of wax. Also we may observe, that at the very beginning of a hive, the bees seldom bring in any substance on their legs for two or three days, and after that, the farina gatherers begin to increase; for now some cells are formed to hold it as a store, and some eggs are laid, which, when hatched, will require this substance as food, and which will be ready when the weather is wet.

"The wax is formed by the bees themselves; it may be called an external secretion of oil, and I have found that it is formed between each scale of the under side of the belly. When I first observed this substance, in my examination of the working bee, I was at a loss to say what it was: I asked myself if it were scales forming, and whether they cast the old, as the lobster, etc. does? but it was to be found only between the scales on the lower side of the belly. On examining the bees through glass hives, while they were climbing up the glass, I could see that most of them had this substance, for it looked as if the lower or posterior edge of the scale was double, or that there were double scales; but I perceived it was loose, not attached. Finding that the substance brought in on their legs was farina, intended, as appeared from every circumstance, to be the food of the bee, and not to make wax; and not having yet perceived any thing that could give me the least idea of wax; I conceived these scales might be it, at least I thought it necessary to investigate them. I therefore took several on the point of a needle, and held them to a candle, where they melted, and immediately formed themselves into round globules; upon which I no longer doubted that this was the wax, which opinion was confirmed to me by not finding those scales but in the building season.

"The cells, or rather the congeries of cells, which compose the comb, may be said to form perpendicular plates, or partitions, which extend from top to bottom of the cavity in which they build, and work downwards; but if the upper part of this vault to which their combs are fixed, is removed, and a dome is put over, they begin at the upper edge of the old comb, and work up into the new cavity at the top. They generally may be guided, as to the directions of their new plates, by forming ridges at top, to which they begin to attach their combs. In a long hive, if these ridges are longitudinal, their plates of comb will be longitudinal; if placed transversely, so will be the plates; and if obliquely, the plates of comb will be oblique also. Each plate consists of a double set of cells, whose bottoms form the partition between each set. The plates themselves are not very regularly arranged, not forming a regular plane where they might have done so, but are often adapted to the situa-tici or shape of the cavity in which they are built

"The bees do not endeavour to shape their cavity to their work, as the wasps do, nor are the cells of equal depths, also fitting them to their situation; but as the breeding cells must all be of a given depth, they reserve a sufficient number for breeding in, and they put the honey into the others, as also into the shallow ones. The attachment of the comb round the cavity is not continued, but interrupted, so as to form passages in the middle of the plates, especially if there be a cross-stick to support the comb; these allow of bees to go across from plate to plate. The substance which they use for attaching their combs to surrounding parts, is not the same as the common wax; it is softer and tougner, a good deal like the substance with which they cover in their chrysalis, or the humblebee surrounds her eggs. It is probably a mixture of wax with farina. The cells are placed nearly horizontal, but not exactly so; the mouth raised a little, which probably may be to retain the honey the better: however, this rule is not strictly observed, for often they are horizontal, and towards the lower edge of a plane of comb they are often declining. The first combs that a hive forms are the smallest, and much neater than the last or lowermost. Their sides or partitions, between cell and cell, are much thinner, and the hexagon is much more perfect. The wax is purer, being probably little else but wax, and it is more brittle. The lower combs are considerably larger, and contain much more wax, or perhaps, more properly, more materials; and the cells are at such distances as to allow them to be of a round figure ; the wax is softer, and there is something mixed with it. I have observed that the cells are not all of equal size, some being a degree larger than others; and that the small are the first formed, and of course at the upper part, where the bees begin; and the larger are nearer the lower part of the comb, or last made : however, in hives of a particular construction, where the bees may begin to work at one end, and can work both down and towards the other end, we often find the larger cells both on the lower part of the combs, and also at the opposite end; these are formed for the males to be bred in: in the hornet and wasp combs there are larger cells for the queens to be bred in; these are also formed in the lower tier, and are the last formed.

"The first comb made in a hive is all of one colour, viz. almost white; but is not so white towards the end of the season, having then more of a yellow cast."