This section is from the book "A Library Of Wonders And Curiosities Found In Nature And Art, Science And Literature", by I. Platt. Also available from Amazon: A library of wonders and curiosities.
M. Huber relates some experiments which confirm the singular discovery of M. Riems, concerning common working bees that are capable of laying eggs, - which, we may remark, is certainly a most convincing proof of their being of the female sex. Eggs were observed to increase in number daily, in a hive in which there were no queens of the usual appearance; but small queens considerably resemble workers, and to discriminate them, required minute inspection. "My assistant," (says M. Huber,) then offered to perform an operation that required both courage and patience, and which I could not resolve to suggest, though the same expedient had occurred to myself. He proposed to examine each bee in the hive separately, to discover whether some small queen had not insinuated herself among them, and escaped our first researches. It was necessary, therefore, to seize every one of the bees, notwithstanding their irritation, and to examine their specific character with the utmost care. This my assistant undertook, and executed with great address. Eleven days were employed in it; and, during all that time, he scarcely allowed himself any relaxation but what the relief of his eyes required. He took every bee in his hand; he attentively examined the trunk, the hind limbs, and the sting; and he found that there was not one without the characteristics of the common bee, that is, the little basket on the hind legs, the long trunk, and the straight sting."
When a supernumerary queen is produced in a hive, or is introduced into it in the course of experiment, either she or the rightful owner soon perishes. The German naturalists, Schirach and Riems, imagined that the working bees assailed the stranger, and stung her to death. Reaumur considered it as more probable, that the sceptre was made to depend on the issue of a single combat between the claimants; and this conjecture is verified by the observations of Huber. The same hostility towards rivals, and destructive vengeance against royal cells, animates all queens, whether they be virgins, or in a state of impregnation, or mothers of numerous broods. The working bees, it may here be remarked, remain quiet spectators of the destruction, by the first-hatched queen, of the remaining royal cells; they approach only to share in the plunder presented by their havock-making mistress, greedily devouring any food found at the bottom of the cells, and even sucking the fluid from the abdomen of the nymphs be fore they toss out the carcase.
The following fact, connected with this subject, is one of the most curious perhaps in the whole history of this wonderful insect. Whenever the workers perceive that there are two rival queens in the hive, numbers of them crowd around each; they seem to be perfectly aware of the approaching deadly conflict, and willing to prompt their Amazonian chieftains to the battle; for as often as the queens show a disinclination to fight, or seem inclined to recede from each other, or to fly off, the bees immediately surround and detain them; but when either combatant shows a disposition to approach her antagonist, all the bees forming the clusters instantly give way, to allow her full liberty for the attack. It seems strange that those bees, who in general show so much anxiety about the safety of their queen, should, in particular circumstances, oppose her preparations to avoid impending danger, - should seem to promote the battle, and to excite the fury of the combatants.
When a queen is removed from a hive, the bees do not immediately perceive it; they continue their labours, "watch over their young, and perform all their ordinary occupations. But, in a few hours, agitation ensues; all appears a scene of tumult in the hive. A singular humming is heard; the bees desert their young, and rush over the surface of the combs with a delirious impetuosity."They have now evidently discovered that their sovereign is gone; and the rapidity with which the bad news spreads through the hive, to the opposite side of the combs, is very remarkable. On replacing the queen in the hive, tranquillity is almost instantly restored. The bees, it is worthy of notice, recognize the individual person of their own queen. If another be palmed upon them, they seize and surround her, so that she is either suffocated, or perishes by hunger; for it is very remarkable, that the workers are never known to attack a queen bee with their stings. If, however, more than eighteen hours have elapsed before the stranger queen be introduced, she has some chance to escape : the bees at first seize and confine her, but less rigidly; and they soon begin to disperse, and at length leave her to reign over a hive, in which she was at first treated as a prisoner, if twenty-four hours have elapsed, the stranger will be well received from the first, and at once admitted to the sovereignty of the hive. In short, it appears that the bees, when deprived of their queen, are thrown into great agitation; that they wait about twenty hours, apparently in hopes of her return; but that, after this interregnum, the agitation ceases, and they set about supplying their loss by beginning to construct royal cells. It is when they are in this temper, and not sooner, that a stranger queen will be graciously received; and upon her being presented to them, the royal cells, in whatever state of forwardness they may happen to be, are instantly abandoned, and the larvae destroyed. Reaumur must therefore have mistaken the result of his own experi ments, when he asserts, that a stranger queen is instantly well received, though presented at the moment when the other is withdrawn. He had seen the bees crowding around her at the entrance of the hive, and laying their antennas over her and this he seems to have taken for caressing. The structure of the hives he employed prevented him from seeing further; had he used the leaf-hive, or one of similar construction, he would have perceived that the apparent caresses of the guards were only the prelude to actual imprisonment
 
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