Coloring matters used with foods are usually soluble in water. If the food under examination be a liquid, it may therefore be treated directly by the method given below. If it be a solid or a pasty substance, soluble in water either in the cold or after heating, it may be dissolved in sufficient water to form a thin liquid. If it contains some insoluble material, it may be treated with sufficient water to dissolve the soluble portion with the formation of a thin liquid and filtered, and then strained through a clean white cotton cloth to separate the insoluble portion. About a half teacupful of the liquid thus described is heated to boiling, after adding a few drops of hydrochloric acid and a small piece of white woolen cloth or a few strands of white woolen yarn. (Before using, the wool should be boiled with water containing a little soda, to remove any fat it may contain, and then washed with water.) The wool is again washed, first with hot and then with cold water, the water pressed out as completely as possible, and the color of the fabric noted. If no marked color is produced, the test may be discontinued and the product considered free from artificial colors. If the fabric is colored, it may have taken up coal-tar colors, some foreign vegetable colors, and if a fruit product is being examined, some of the natural coloring matter of the fruit. Rinse the fabric in hot water, and then boil for 2 or 3 minutes in about one-third of a teacupful of water and 2 or 3 teaspoonfuls of household ammonia. Remove and free from as much of the liquid as possible by squeezing or wringing. Usually the fabric will retain the greater part of the natural fruit color, while the coal-tar color dissolves in dilute ammonia. The liquid is then stirred with a splinter of wood and hydrochloric acid added, a drop or two at a time, until there is no longer any odor of ammonia. (The atmosphere of the vessel is sometimes charged with the ammonia for several minutes after it has all been driven out of the liquid; therefore one should blow into the dish to remove this air before deciding whether the ammonia odor has been removed or not.) When enough acid has been added the liquid has a sour taste, as may be determined by touching the splinter, used in stirring, to the tongue.

A fresh piece of white woolen cloth is boiled in this liquid and thoroughly washed. If this piece of cloth has a distinct color the food under examination is artificially colored. The color used may have been a coal-tar derivative, commonly called an aniline dye, or an artificial color chemically prepared from some vegetable color. If of the first class the dyed fabric is usually turned purple or blue by ammonia. In either case, if the second fabric has a distinct color, it is evident that the product under examination is artificially colored. Of course a dull, faint tint must be disregarded.