In regard to other circumstances relative to the presence of this fungus, there are, above all, two remarkable facts, namely, its property of adhering to surfaces as perfectly polished as that of a mirror, and its power of resistance against the reagents, if we except the caustic alkalies and the concentrated mineral acids. This power of resisting the ordinary reagents explains in a plausible manner why the fungus is not destroyed by the digestive process in the stomach, where, however, the acid reaction of the gastric juice probably arrests its development--is that of the schistomycetes in general--and keeps it in a state of temporary inactivity. This property of adhering to smooth surfaces explains perhaps the power of the Eucalyptus globulus in arresting the progress of paludal miasm (?). But it is evident that other trees, shrubs, and plants of resinous or balsamic foliage, as, for example, the Populus balsamifera, Cannabis sativa, Pinus silvestris, Pinus abies, Juniperus communis, have equally, with us, the same faculty; they are favorable also for the drying of the soil, and the more completely, as their roots are spreading, more extended, and more ramified.

In order to demonstrate the presence of the limnophysalis in the blood of patients affected with intermittent fever during the febrile stage, properly speaking, it appeared necessary for me to dilute the blood of patients with a solution of nitrate of potassa, having at 37.5°C. the same specific gravity as the serum of the blood. With capillary tubes of glass, a little dilated toward the middle, of the same shape and size as those which are used in collecting vaccine lymph, I took up a little of the solution of nitrate of potassa above indicated. After this I introduced the point of an ordinary inoculating needle under the skin, especially in the splenic region, where I ruptured some of the smallest blood-vessels of the subcutaneous cellular tissue. I collected some of the blood which flowed out or was forced out by pressure, in the capillary tubes just described, containing a solution of potassa; after which I melted the ends with the flame of a candle. With all the intermittent fever patients whose blood I have collected and diluted during the febrile stage, properly speaking, I have constantly succeeded in finding the Limnophysalis hyalina in the blood by microscopic examination.

It is only necessary for me to mention here that it is of the highest importance to be able to demonstrate the presence of fungus in the blood of the circulation and in the urine of patients in whom the diagnosis is doubtful. The presence of the Limnophysalis hyalina in the urine indicates that the patient is liable to a relapse, and that his intermittent fever is not cured, which is important in a prognostic and therapeutic point of view.

When the question is to prevent the propagation of intermittent fevers, it is evident that it should be remembered that the Limnophysalis hyalina enters into the blood by the mucous membrane of the organs of respiration, of digestion, and the surface of the pulmonary vesicles. We have also to consider the soil, and the water that is used for drinking.

In regard to the soil, several circumstances are very worthy of attention. It is desirable, not only to lower as much as possible the level of the subterranean water (grunawassen) by pipes of deep drainage, the cleansing, and if there is reason, the enlargement (J. Ory) of the capacity of the water collectors, besides covering and keeping in perfect repair the principal ditches in all the secondary valleys to render the lands wholesome, but also to completely drain the ground, diverting the rain water and cultivating the land, in the cultivation of which those trees, shrubs, and plants should be selected which thrive the most on marshy grounds and on the shores and paludal coasts of the sea, and which have their roots most speading and most ramified. Some of the ordinary grasses are also quite appropriate, but crops of the cereals, which are obtained after a suitable reformation of marshy lands, yield a much better return. After the soil in the neighborhood of the dwellings has been drained and cultivated with care, and in a more systematic manner than at present, the bottoms of the cellars should be purified as well as the foundations of the walls and of the houses.

The water intended for drinking, which contains the Limnophysalis hyalina, should be freed from the fungus by a vigorous filtration. But, as it is known, the filtering beds of the basins in the water conduits are soon covered with a thick coating of confervae, and the Limnophysalis hyalina then extends from the deepest portions of the filtering beds into the filtered water subjacent. It is for this reason that it is absolutely necessary to renew so often the filtering beds of the water conduits, and, at all events, before they have become coated with a thick layer of confervae. The disappearance of intermittent fevers will testify to the utility of these measures. It is for a similar reason that wooden barrels are so injurious for equipages. When the wood has begun to decay by the contact of the impure water, the filaments of mycelium of the Limnophysalis hyalina penetrate into the decayed wood, which becomes a fertile soil for the intermittent fever fungi.

The employment for the preparation of mortar of water not filtered, or of foul, muddy sand which contains the Limnophysalis hyalina, explains how intermittent fevers may proceed from the walls of houses. This arises also from the pasting of wall-paper with flour paste prepared with water which contains an abundance of the fungi of intermittent fever.

The miasm in the latter case is therefore endoecic, or more exactly entoichic. With us the propagation of intermittent fever has been observed in persons occupying rooms scoured with unfiltered water containing the Limnophysalis hyalina in great quantity.