At 6 A.M. collected and examined for specimens the drops of dew deposited. Results: In every one of the five instances collected the automobile spores, and the sporangia of the gemiasmas and the protuberans on both sides of slides and beaker. There were also spores and mycelial filaments of fungi, dirt, and zoospores. The drops of dew were collected with capillary tubes such as were used in Edinburgh for vaccine virus. The fluid was then preserved and examined in the naval laboratory. In a few hours the spores disappeared.
Observation 32. Some of the earth near the site of the exposure referred to in Observation 31, was examined and found to contain abundantly the Gemiasma verdans, rubra, Protuberans lamella, confirmed by three more observations.
Observation 33. In company with Surgeon F. M. Dearborne, U.S.N., in charge of Naval Hospital, the same day later explored the wall about marsh west of hospital. Found the area abundantly supplied with palmellae, Gemiasma rubra, verdans, and Protuberans lamella, even where there was no incrustation or green mould. Made very many examinations, always finding the plants and spores, giving up only when both of us were overcome with the heat.
Observation 34. August, 1881. Visited the Wallabout; found it filled up with earth. August 17. Visited the Flushing district; examined for the gemiasma the same localities above named, but found only a few dried up plants and plenty of spores. With sticks dug up the earth in various places near by. Early in September revisited the same, but found nothing more; the incrustation, not even so much as before. The weather was continuously for a long time very dry, so much so that vegetables and milk were scarce.
The grass and grounds were all dried up and cracked with fissures.
There must be some moisture for the development of the plants. Perhaps if I had been able to visit the spots in the early morning, it would have been much better, as about the same time I was studying the same vegetation on 165th Street and 10th Avenue, New York, and found an abundance of the plants in the morning, but none scarcely in the afternoon.
Should any care to repeat these observations, these limits should be observed and the old adage about "the early bird catching the worm," etc. Some may object to this directness of report, and say that we should report all the forms of life seen. To this I would say that the position I occupy is much different from yours, which is that of discoverer. When a detective is sent out to catch a rogue, he tumbles himself but little with people or things that have no resemblance to the rogue. Suppose he should return with a report as to the houses, plants, animals, etc., he encountered in his search; the report might be very interesting as a matter of general information, but rather out of place for the parties who desire the rogue caught. So in my search I made a special work of catching the gemiasmas and not caring for anything else. Still, to remove from your mind any anxiety that I may possibly not have understood how to conduct my work, I will introduce here a report of search to find out how many forms of life and substances I could recognize in the water of a hydrant fed by Croton water (two specimens only), during the present winter (1881 and 1882) I beg leave to subjoin the following list of species, not individuals, I was able to recognize.
In this list you will see the Gemiasma verdans distinguished from its associate objects. I think I can in no other way more clearly show my right to have my honest opinion respected in relation to the subject in question.
MALARIA PLANTS COLLECTED SEPT. 10, 1882, AT WASHINGTON HEIGHTS, 176TH STREET, NEAR 10TH AVENUE, NEW YORK CITY, ETC.
PLATE VIII.--A, B, C, Large plants of Gemiasma verdans. A, Mature plant. B, Mature plant discharging spores and spermatia through a small opening in the cell wall. C, A plant nearly emptied. D, Gemiasma rubra; mature plant filled with microspores. E, Ripe plant discharging contents. F, Ripe plant, contents nearly discharged; a few active spermatia left behind and escaping. G, nearly empty plant. H, Vegetation in the SWEAT of ague cases during the paroxysm of sweating. I, Vegetation in the BLOOD of ague. J, Vegetation in the urine of ague during paroxysm. K, L, M, Vegetation in the urine of chronic cases of severe congestive type. N, Vegetation in BLOOD of Panama fever; white corpuscles distended with spores of Gemiasma. O, Gemiasma alba. P, Gemiasma rubra. Q, Gemiasma verdans. R, Gemiasma alba. O, P, Q, R, Found June 28,1867, in profusion between Euclid and Superior Streets, near Hudson, Cleveland, O. S, Sporangia of Protuberans.
List of objects found in the Croton water, winter of 1881 and 1882. The specimens obtained by filtering about one barrel of water:
1. Acineta tuberosa. 2. Actinophrys sol. 3. Amoeba proteus. 4. " radiosa. 5. " verrucosa. 6. Anabaina subtularia. 7. Ankistrodesmus falcatus. 8. Anurea longispinis. 9. " monostylus. 10. Anguillula fluviatilis. 11. Arcella mitrata. 12. " vulgaris. 13. Argulus. 14. Arthrodesmus convergens. 15. Arthrodesmus divergens. 16. Astrionella formosa. 17. Bacteria. 18. Bosmina. 19. Botryiococcus. 20. Branchippus stagnalis. 21. Castor. 22. Centropyxis. 23. Chetochilis. 24. Chilomonads. 25. Chlorococcus. 26. Chydorus. 27. Chytridium. 28. Clatbrocystis aeruginosa. 29. Closterium lunula. 30. " didymotocum. 31. " moniliferum. 32. Coelastrum sphericum. 33. Cosmarium binoculatum. 34. Cyclops quad. 35. Cyphroderia amp. 36. Cypris tristriata. 37. Daphnia pulex. 38. Diaptomas castor. 39. " sull. 40. Diatoma vulgaris. 41. Difflugia cratera. 42. " globosa. 43. Dinobryina sertularia. 44. Dinocharis pocillum. 45. Dirt. 46. Eggs of polyp. 47. " entomostraca. 48. " plumatella. 49. " bryozoa. 50. Enchylis pupa. 51. Eosphora aurita. 52. Epithelia, animal. 53. " vegetable. 54. Euastrum. 55. Euglenia viridis.