But the point of difficulty was B. termo. The demonstration of its flagella was a task of difficulty which only patient purpose could conquer. But by the use of our new lenses, and special illumination we - my colleague and I - were enabled to demonstrate clearly a flagellum at each end of this least of living organisms, as you see, and by the rapid lashing of the fluid, alternately or together, with these flagella, the powerful, rapid, and graceful movements of this smallest known living thing are accomplished. Of course these fibers are inconceivably fine - indeed for this very reason it was desirable, if possible, to measure it, to discover its actual thickness. We all know that, both for the telescope and the microscope, beautiful apparatus are made for measuring minute magnified details. But unfortunately no instrument manufactured was delicate enough to measure directly this fiber. If it were measured it must be by an indirect progress, which I accomplished thus: The diameter of the body of B. termo, i.e., from; side to side, may in different forms vary from the 1/20000 to the 1/50000 of an inch. That is a measurement which we may easily make directly with a micrometer.

Haying ascertained this, I determined to discover the ratio of thickness between the body of the Bacterium and its flagellum - that is to say, to discover how many of the flagella laid side by side would make up the width of the body.

I proceeded thus: This is a complicated microscope placed on a tripod, so arranged that it may be conveniently worked upright. There is a special instrument for centering and illuminating. On the stage of the instrument, the Bacterium with its flagellum in distinct focus is placed. Instead of the simple eyepiece, camera lucida is placed upon it. This instrument is so constructed that it appears to throw the image of the object upon the white sheet of paper on the small table at the right hand where the drawing is made, at the, same time that it enables the same eye to see the pencil and the right hand. In this way I made a careful drawing of B. termo and its flagellum, magnified 5,000 diameters. Here is a projection of the drawing made. But I subsequently avoided paper, and used under the camera most carefully prepared surface of ground glass. When the drawing was made I placed on the drawing a drop of Canada balsam, and covered it with a circle of thin glass, just like any other microscopic mounted object. This is a micro-slide so prepared. Now you can see that I only have to lay this on the stage of a microscope, make it an object for a low power, and use a screw micrometer to find how many flagella go to the making of a body.

The result is given in the figure; you see that ten flagella would fill the area occupied by the diameter of the body.

In the case chosen the body was the 1/20,400 of an inch wide, and therefore, when divided by ten, gave for the flagellum a thickness of the 1/204,000 of an English inch. In the end I made fifty separate drawings with four separate lenses. I averaged the result in each fifty, and then took the average of the total of 200, and the mean value of the width of the flagellum was the 1/204,700 of an English inch. It will be seen, then, that we are possessed of instruments which, when competently used, will enable us to study the life-histories of the putrefactive organisms, although they are the minutest forms of life. I have stated that they were the inevitable accompaniments of putrescence and decay. You learned from a previous illustration the general appearance of the Bacteria; they are the earliest to appear whenever putrefaction shows itself. In fact the pioneer is this - the ubiquitous Bacterium termo. The order of succession of the other forms is by no means certain. But whenever a high stage of decomposition is reached, a group of forms represented by these three will swarm the fluid.

These are the Monads, they are strictly putrefactive organisms, they are midway in size between the least and largest Bacteria, and are, from their form and other conditions, more amenable to research, and twelve years ago I resolved, with the highest power lenses and considerable practice in their use, to attack the problem of their origin; whether as physical products of the not-living, or as the natural progeny of parents.

But you will remember that only a minute drop of fluid containing them can be examined at one time. This minute drop has to be covered with a minute film of glass not more than the 1/200 of an inch thick. The highest lenses are employed, working so near as almost to touch the delicate cover. Clearly, then, the film of fluid would rapidly evaporate and cause the destruction of the object studied. To prevent this an arrangement was devised by which the lens and the covered fluid under examination were used in an air-tight chamber, the air of which was kept in a saturated condition; so that being, like a saturated sponge, unable to take in any more, it left the film of fluid unaffected. But to make the work efficient I soon found that there must be a second observer. Observation by leaps was of no avail. To be accurate it must be unbroken. There must be no gap in a chain of demonstration. A thousand mishaps would occur in trying to follow a single organism through all the changes of successive hours to the end. But, however many failures, it was evident, we must begin on another form at the earliest point again, and follow it to the close. I saw soon that every other method would have been merely empirical, a mere piecemeal of imagination and fact.

When one observer's ability to continue a long observation was exhausted, there must be another at hand to take up the thread and continue it; and thus to the end. I was fortunate indeed at this time in securing the ready and enthusiastic aid of Dr. J.J. Drysdale, of Liverpool, who practically lived with me for the purpose, and went side by side with me to the work. We admitted nothing which we had not both seen, and we succeeded each other consecutively, whenever needful, in following to the end the complete life-histories of six of these remarkable forms.