This section is from "Scientific American Supplement". Also available from Amazon: Scientific American Reference Book.
I will now give you the facts in relation to two which shall be typical. We obtained them in enormous abundance in a maceration of fish. I will not take them in the order of our researches, but shall find it best to examine the largest and the smallest. The appearance of the former is now before you. It is divergent from the common type when seen in its perfect condition, avoiding the oval form, but it resumes it in metamorphosis. It is comparatively huge in its proportions, its average extreme length being the 1/1000 of an inch. Its normal form is rigidly adhered to as that of a rotifer or a crustacean. Its body-substance is a structureless sarcode. Its differentiations are a nucleus-like body, not common to the monads; generally a pair of dilating vacuoles, which open and close, like the human eyelid, ten to twenty times in every minute; and lastly, the usual number of four flagella. That the power of motion in these forms and in the Bacteria is dependent upon these flagella I believe there can be no reasonable doubt. In the monads, the versatility, rapidity, and power of movement are always correlated with the number of these. The one before us could sweep across the field with majestic slowness, or dart with lightning swiftness and a swallow's grace.
It could gyrate in a spiral, or spin on its axis in a rectilinear path like a rifled bullet. It could dart up or down, and begin, arrest, or change its motion with a grace and power which at once astonish and entrance. Fixing on one of these monads then, we followed it doggedly by a never-ceasing movement of a "mechanical stage," never for an instant losing it through all its wanderings and gyrations; We found that in the course of minutes, or of hours, the sharpness of its outline slowly vanish, its vacuoles disappeared, and it lost its sharp caudal extremity, and was sluggishly amoeboid. This condition tensified, the amoeboid action quickened as here depicted, the agility of motion ceased, the nucleus body became strongly developed, and the whole sarcode was in a state of vivid and glittering action.
If now it be sharply and specially looked for, it will be seen that the root of the flagella splits, dividing henceforth into two separate pairs. At the same moment a motion is set up which pulls the divided pairs asunder, making the interval of sarcode to grow constantly greater between them. During this time the nuclear body has commenced and continued a process of self-division; from this moment the organism grows rapidly rounder, the flagella swiftly diverge. A bean-like form is taken; the nucleus divides, and a constriction is suddenly developed; this deepens; the opposite position of the flagella ensues, the nearly divided forms now vigorously pull in opposite directions, the constriction is thus deepened and the tail formed. The fiber of sarcode, to which the constricted part has by tension been reduced, now snaps, and two organisms go free. It will have struck you that the new organism enters upon its career with only two flagella, and the normal organism is possessed of four. But in a few minutes, three or four at most, the full complement were always there. How they were acquired it was the work of months to discover, but at last the mystery was solved.
The newly-fissioned form darted irregularly and rapidly for a brief space, then fixed itself to the floor or to a rigid object by the ends of its flagella, and, with its body motionless, an intense vibratory action was set up along the entire length of these exquisite fibers. Rapidly the ends split, one-half being in each fiber set free, and the other remaining fixed, and in 130 seconds each entire flagellum was divided into a perfect pair.
Now the amoeboid state is a notable phenomenon throughout the monads as precursive of striking change. It appears to subserve the purpose of the more facile acquisition and digestion of food at a crisis. And this augmented the difficulty of discovering further change; and only persistent effort enabled us to discover that with comparative rareness there appeared a form in an amoeboid state that was unique. It was a condition chiefly confined to the caudal end, the sarcode having became diffluent, hyaline, and intensely rapid in the protrusion and retraction of its substance, while the nuclear body becomes enormously enlarged. These never appear alone; forms in a like condition are diffused throughout the fluid, and may swim in this state for hours. Meanwhile, the diffluence causes a spreading and flattening of the sarcode and swimming gives place to creeping, while the flagella violently lash. In this condition two forms meet by apparent accident, the protrusions touch, and instant fusion supervenes. In the course of a few seconds there is no disconnected sarcode visible, and in five to seven minutes the organism is a union of two of the organisms, the swimming being again resumed, the flagella acting in apparent concert.
This may continue for a short time, when movement begins to flag and then ceases. Meanwhile, the bodies close together, and the eyenots or vacuoles melt together, the two nuclei become one and disappear, and in eighteen hours the entire body of "either has melted into other," and a motionless, and for a time irregular, sac is left. This now becomes smooth, spherical, and tight, being fixed and motionless. This is a typical process; but the mingled weariness and pleasure realized in following such a form without a break through all the varied changes into this condition is not easily expressed.
But now the utmost power of lenses, the most delicate adjustment of light, and the keenest powers of eyesight and attention must do the rest. Before the end of six hours the delicate glossy sac opens gently at one place, then there streams out a glairy fluid densely packed with semi-opaque granules, just fairly visible when their area was increased six millions of times, and this continued until the whole sac was empty and its entire contents diffused. To follow with our utmost powers these exquisite specks was an unspeakable pleasure, a group seen to roll from the sac, when nearly empty, were fixed and never left. They soon palpably changed by apparent swelling or growth, but were perfectly inactive; but at the end of three hours a beaked appearance was presented. Rapid growth set in, and at the end of another hour, how has entirely baffled us, they acquired flagella and swam freely; in thirty-five minutes more they possessed a nucleus and rapidly developed, until at the end of nine hours after emission a sporule was followed to the parent condition and left in the act of fission.
 
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