The research began with the fundamental question of the selection of subjects, the various requirements and degree of personal integrity demanded of the men limiting our field of selection greatly. Pending the actual selection of subjects, a large amount of preliminary work was done in getting together apparatus for studying the respiratory exchange and gas analysis and planning for the transportation of a considerable amount of material to Springfield. As previously stated, the selection of men was based in part upon physical qualifications and intellectual ability, and none of the men in Squad A were accepted until Dr. Chapin had been convinced that they were in good physical condition, according to clinical standards. This squad was placed at a special table and allowed to eat ad libitum for several days, so that the normal food consumption of the men prior to restriction might be determined. In this preliminary period the food was carefully ali-quoted, sampled, and prepared for analysis. The following instructions were given Squad A and in somewhat revised form to Squad B:

The highly scientific character of this research and its magnitude are such as to demand from all connected with the work, either as subjects or as assistants and collaborators, an honest, faithful and accurate service ever maintained above reproach.

1. The subject must be in good health and confident of continued good physical condition under usual conditions of living.

2. He will sign an affidavit of his willingness to subscribe to all the requirements of the research.

3. He will promise uninterrupted service and cooperation during the entire period of observation and will not seek to be released from his responsibilities as a subject until the completion of the research unless compelled to do so by major causes, such causes to be declared justifiable by the squad physician (a Springfield physician) or by a committee of the faculty. This period is to begin on Wednesday, Sept. 26, 1917, and end by Christmas, 1917.

4. Provision is to be made for the possibility of continuing the observations from 2 to 3 weeks longer if necessary. As this is likely to interfere seriously with the plans of some subjects during the Christmas vacation, it is possible that some way may be provided to meet the requirements of the research and still allow subjects to absent themselves during the usual holiday period.

5. The subjects will conform to the usual modes of living as regards work, exercise, diet (except as restricted) and sleep. No radical change in the ordinary daily life of the subject is permitted.

6. The subject will submit to a reduction in the daily food allowance sufficient to induce a loss of 10 per cent or more of body-weight in as short a time as possible, and also to maintain this acquired reduction in weight until the end of the test period.

7. The food will be served at a table reserved for the squad of subjects in Woods Hall dining room and will consist of the regular bill of fare for all students of the college.

8. It is desired that all portions of the food served be eaten completely. If not, the person in charge of the serving must be notified and will make a record of the food not eaten and, if necessary, collect it for analysis. It is supposed that when the ration is reduced the subjects will have no difficulty in eating the entire ration served.

9. The subject will not eat or drink anything whatsoever outside of the regular three meals served under supervision.

10. Water only in reasonable quantity is allowed at any time.

11. The subject must clearly understand that under the conditions of the research, the partaking of ice cream, fruit, peanuts, popcorn, etc., as well as sodas or drinks of any kind, unless intentionally served as a part of the regular allowance, is absolutely prohibited. The moderate chewing of gum is not prohibited.

12. It must, however, be understood that any breaking of this most important rule should be promptly reported, as by so doing the seriousness of the offense can be in part minimized.

13. Collection Of The Urine

(a) All urine voided will be continuously collected for exact periods of 24 hours each in properly labeled bottles which will be provided for the purpose. (6) Each 24-hour collection will be completed daily at 5h30m a. m. and placed at the disposal of the collector in charge at the place indicated for this, (c) No urine should be lost. Accidental losses must be prevented. If any occur they must be carefully noted and the quantity lost reported as accurately as possible the same day. (d) The loss of urine during defecation will be prevented by the simultaneous use of a urine-collecting bottle, (e) If urination is to occur away from the college premises the subject must take with him a bottle to receive the urine passed, which is to be added to the day's collection. (f) The collection of urine at the time the squad goes to Boston will not be interrupted until Sunday morning. Provision for this must be made as needed. The collection of urine on the Sundays in Boston will be omitted according to instructions given elsewhere.

14. Collection Of Feces During Weekly Digestion Period

(a) Each Monday morning at breakfast with the first mouthfuls of food eaten, two or three gelatin capsules containing charcoal or carmine are to be swallowed. They will likewise be given with the first meal on Thursday morning. (6) Collect all the stools from Monday until the collector in charge will have notified you that the charcoal given on Thursday morning has made its appearance in the stools. Under ordinary circumstances this will occur on Friday or perhaps not until Saturday.

15. Trips To Boston

(a) On Saturday afternoon, Sept. 29, and every two weeks hereafter, the squad will be taken to Boston. (6) On arriving a regulation supper will be served at a restaurant and the squad then conducted to the Nutrition Laboratory, (c) The evening will be spent in making various tests and taking measurements on the subjects, (d) The squad will retire and sleep in a large chamber constructed for the purpose of estimating the basal metabolism of its occupants, (e) Breakfast will be served at the laboratory on Sunday morning, after which the squad will be free to spend the day as it desires with the understanding that: (1) A low ration diet will be adhered to at noon and evening. (2) No urine need be collected from 5h30m a. m. Sunday until 5h30m a. m. Monday. This period, in which urine is not to be collected, ends with and includes the emptying of the bladder at exactly 5h30m a. m. each Monday. The first passage of urine to be again collected will be the first urination following that of 5h30m a. m. which is discarded only on each Monday morning.