Fifty c.c. of the wine, contained in a flask of about 200 c.c. capacity, are distilled in a current of steam,

The operation is so arranged as to allow the volume of the wine to be rapidly reduced to about 25 c.c., after which it is kept constant, and the distillation terminated when the volume of the distillate is about 200 c.c. This is titrated with N/10.alkali, using phenol. phthalein as indicator, and the result expressed in terms of acetic acid. One c.c. of N/10.alkali = 0.006 gram of acetic acid.