Chapter 12, Note 4. Quenching Action And Anti Carcinogenic Effect Of Conjugated Fatty Acids
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This section is from the book "Research In Physiopathology As Basis Of Guided Chemotherapy With Special Application To Cancer", by Emanuel Revici. Also available from amazon: Research In Physiopathology
Chapter 12, Note 4. Quenching Action And Anti Carcinogenic Effect Of Conjugated Fatty Acids
We have investigated the influence exerted by different fatty acids, conjugated and nonconjugated, upon various carcinogens. In a first group of experiments we studied this influence in vitro, and chose the quenching of the fluorescence of the carcinogen as criterion of activity.
This part of the research was made in collaboration with C. Huesca Mejia and P. Teitelbaum.
The quenching effect has been studied as follows:
The fluorescence of carcinogenic hydrocarbons is measured by means of the fluorescent attachment to the Beckman spectrophotometer using a wave length around 365 mµ. The sensitivity of the apparatus is adjusted to show a value of 100 for the fluorescent light utilizing the concentration of the hydrocarbon having the maximum fluorescence. The carcinogenic hydrocarbons are dissolved in alcohol, iso octans or cyclohexane, the last two being purified and thus rendered optically inactive by passage through a silica column.
The fatty acids are dissolved in varying dilutions in the same solvents and added to the solution of hydrocarbon carcinogen which has been previously chosen to give a fluorescent value of 100. The fluorescence of the mixture is immediately determined. The quenching effect of the fatty acid is shown by the percent of the residual fluorescence of the carcinogen when mixed with different solutions of a mixture of conjugated fish oil fatty acids. Table XXXVI shows the quenching effect of mixtures of fatty acids conjugated by treatment with KOH upon different carcinogenic and related hydrocarbons.
Fig. 280. Conjugation method. The use of ethylic alcohol as solvent for conjugation changes the amount of polyconjugated members obtained for cod liver oil fatty acids (a) as compared with those obtained when the conjugation is made at higher temperatures using ethyleneglycol, (b) glycerol or other solvents. Dilution 0.002% in ethyl alcohol.
Table XXXVI. Quenching Effect Of Conjugated Fatty Acid Mixtures Upon The Fluorescence Of Carcinogenic And Related Hydrocarbons
Hydrocarbon | % Concentration | Quenching Agent | % Residua Fluorescenc |
Mixture of conjugated fatty acids from cod liver oil | |||
0.0062 | (0.1% solution) | 25.0 | |
3, 4 Benzpyrene | 0.0062 | 36.0 | |
1, 2 Benzanthracene | 0.0031 | " | 24.0 |
1, 2, 5, 6 Dibenzanthracene | Sat. Sol. (alcohol) | " | 15.2 |
9, 10 Dimethyl -1, 2 Benzanthracene | 0.0062 | " | 23.0 |
Mixture of conjugated fatty acids from fish oil | |||
Benzanthracene 12 ol -7 Methylacetate | 0.005 | (0.2% solution) | 5.7 |
7 ChIoro-10 Methyl -1, 2 Benzanthracene | 0.005 | " | 7.9 |
0.005 | " | 10.0 | |
0.005 | 11.2 | ||
0.001 | " | 6.5 | |
7 Cyano-10 Methyl -1, 2 Benzanthracene | 0.0057 | " | 4.0 |
5 Chloro-10 Methyl -1,2 Benzanthracene. | 0.0025 | " | 5.8 |
6 Chloro-10 Methyl -1, 2 Benzanthracene .* | 0.01 | " | 5.2 |
0.005 | " | 13.0 |
* Fluorescence set at 45.
Table XXXVII. Quenching Effect Of Various Nonconjugated And Conjugated Fatty Acid Mixtures Upon The Fluorescence Of Carcinogenic And Related Hydrocarbons
Hydrocarbon | % Concentration | Fatty Acids from | Conjugated Fatty Acids from | B- Eleostearic Acid | Linoleic Acid | Conj. Linoleic Acid | Oleic Acid |
Cod Liver Oil | |||||||
0.1% | 0.1% | 0.1% | 0.1% | 0.1% | 0.1% | ||
Methylcholanthrene | 0.0062 | 92.5 | 25.0 | 95.8 | 95.0 | 89.8 | — |
3, 4 Benzpyrene | 0.0062 | 82.8 | 36.0 | 100.0 | — | — | |
1,2 Benzanthracene | 0.0031 | 93.4 | 24.0 | 94.8 | 100.5 | 97.0 | 100.8 |
1, 2, 5, 6 Dibenzanthracene | Sat. Sol. (alcohol) | 89.0 | 15.2 | 100.0 | 97.0 | 98.0 | 100.0 |
9, 10 Dimethyl -1,2 Benzanthracene | 0.0062 | 90.8 | 23.0 | 97.8 | 93.5 | 100.0 | 96.0 |
Fish Oil | |||||||
0.2% | 0.2% | 0.2% | 0.2% | 0.2% | 0.2% | ||
Benzanthracene 12 ol -7 Methylacetate | 0.005 | 66.0 | 5.7 | 89.0 | 87.0 | 80.0 | 92.1 |
7 Chloro-10 Methyl -1,2 Benzanthracene | 0.005 | 67.0 | 7.9 | 84.0 | 85.0 | 87.0 | 87.0 |
1 Cholanthrene -3 Methyl | 0.005 | 110.0 | 10.0 | 110.0 | 110.0 | 110.0 | 110.0 |
Benzo (d) Pyrene -5 Methyl | 0.005 | 88.0 | 11.2 | 88.0 | 70.0 | 90.0 | 91.0 |
3, 10 Dimethyl -1,2 Benzanthracene | 0.001 | 72.0 | 6.5 | 63.0 | 78.0 | 74.0 | 73.0 |
7 Cyano-10 Methyl -1,2 Benzanthracene | 0.0057 | 83.5 | 4.0 | 91.0 | 90.2 | 88.5 | 91.0 |
5 Chloro-10 Methyl -1,2 Benzanthracene | 0.0025 | 89.0 | 5.8 | 92.0 | 95.0 | 92.0 | 93.5 |
6 Chloro-10 Methyl -1,2 Benzanthracene* | 0.01 | 47.0 | 5.2 | 43.0 | 45.0 | 42.0 | 45.0 |
4 Methoxy -3, 4 Benzpyrene | 0.005 | 88.0 | 13.0 | 86.0 | 90.0 | 89.0 | 91.0 |
* Fluorescence set at 45.
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