Bearing in mind the role of acid lipidic constituents in the physiology of various biological entities, preparations containing these fractions were obtained. Tissues, organs, organisms, and organic products were saponified and acid fractions soluble in ether were isolated. We called them "acid lipidic fractions," or "lipoacidic fractions." Their analyses revealed, in addition to various fatty acids, other substances with lipidic and acid character. Some were identified as porphyrinic acids.

Significant differences related to the sources of these preparations could be recognized in biological effects at different levels. Preparations obtained from intestine, for instance, had no obvious effects on any of the manifestations; there were no systemic or organic changes, no effects upon pain, red cells or leucocytes in vitro, and no influence on tissue respiration. Treatment of successive transplants showed no apparent effects, even after ten passage generations. No effects upon organs through repeated injections of cells treated in vivo by these preparations were seen.

Spectral analysis of rat liver fatty acids

Fig. 120. Spectral analysis of rat liver fatty acids after chemical isomerization shows the presence of fatty acids with 3 and 4 double bonds. (0.002% in ethyl alcohol).

On the other hand, other preparations from placenta, liver, blood, etc. did show activity upon all manifestations, including pain. They induced negative results on tumor growth after transplant dipping for just two or three generations.

The differences in activity could be related to the richness of these preparations in polyunsaturated fatty acids. It could be shown that it was not the total number of double bonds present, as determined by the iodine number, that was significant but rather the relative abundance of higher unsaturated members, as recognized by special analysis after adequate chemical conjugation. Figures 120, 121 and 122 show such analyses.

Spectral analysis of the intestinal fatty acids of rats after chemical isomerization

Fig. 121. Spectral analysis of the intestinal fatty acids of rats after chemical isomerization shows minimal amounts of members with 3, 4 or more double bonds. (0.002% in ethyl alcohol).